Research on development and application of biomarkers for verification of human exposures to chlorine gas

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790651
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Legal medicine
• Research Institution: National Research Institute of Police Science
• Principal Investigator: OHSAWA Isaac 科学警察研究所, 法科学第三部, 主任研究官 (30370886)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 塩素ガス暴露 / バイオマーカー

Outline of Final Research Achievements

3,5-dichlorotyrosine was synthesized, and we developed an analytical method to
detect 3-chlorotyrosine and 3,5-dichlorotyrosine using LC-MS. The mothod uses a general reversed-phase column or a mix-mode column of normal-phase and cation exchange. When we apply the method to normal human urine, chlorinated tyrosines were under detection limit. Although we also apply the method to pooled normal human plasma after hydrolyzing plasma protein with methansulfonic acid, chlorinated tyrosines were also under detection limit. On the other hand, chlorinated tyrosines were detected in the normal human plasma after exposure to chlorine gas. It was also observed that the amount of generated chlorinated tyrosines depends on concentration of chlorine gas exposed to the human plasma.

Free Research Field

法中毒学