2013 Fiscal Year Final Research Report

Investigation of anti-aging effect of Kampo medicine on the skin, targeting stratum corneum

Research Project

Project/Area Number	24790660
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	General internal medicine (including Psychosomatic medicine)
Research Institution	Osaka City University
Principal Investigator	TAMIYA Hisashi 大阪市立大学, 大学院医学研究科, 講師 (80464634)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	漢方 / 皮膚アンチエイジング / 当帰飲子 / 角層ターンオーバー
Research Abstract	We investigated the efficacy of Toki-inshi (TO), one of traditional Japanese kampo prescriptions, for aged skin of mice, targeted on the stratum corneum (SC). Water containing of SC (WCSC) increased significantly in dose-dependent manner by oral TO. In addition, cell areas of corneocytes (CAC) and degrees of multiple detachments (DMD) showed significant reduction dose-dependently. Epidermal thickness of the groups given feds containing more than 2% of TO increased significantly, which was consistent with increase of the number of PCNA-positive basal cells and mRNA level of epiregulin. The expression of filaggrin also increased in the group of 10% of TO in both immunohistochemistry and mRNA level. Oral TO has skin moisturizing effect in the elderly mice, which are associated with the improvement of impaired turnover of SC from aging by activating the proliferation of basal cell, and enhancement of retention ability of skin moisture by increased level of filaggrin.