2013 Fiscal Year Final Research Report
Bone-marrow adipocytes as a new cell source for cell therapy in osteoporosis and aplastic anemia
| Project/Area Number |
24790664
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General internal medicine (including Psychosomatic medicine)
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | DFAT / 脱分化 / 骨髄中間葉系幹細胞 / 骨粗鬆症 / 再生不良性貧血 |
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| Research Abstract |
In BM-DFAT prepared from bone marrow fluid from patients undergoing implant arthroplasty, osteogenic-specific genes were expressed equivalently in BM-MSC prepared from same donor. Alkaline phosphatase was strongly expressed before and during osteogenic differentiation induction, and mineralized matrix aggregates were observed after 2 weeks of differentiation induction by staining for alizarin red S. In a mouse bone fracture model, bone density in BM-DFAT grafts were significantly higher compared with those in control grafts.
In BM-DFAT prepared from bone marrow fluid from patients with aplastic anemia, proteins and mRNA levels for SDF-1 were significantly higher than BM-MSC prepared from same donor. Implantation of these BM-DFAT and human umbilical cord blood (UCB) CD34+ cells into a NOD/SCID mouse model of graft failure of human UCB resulted in a detection of human blood cell fractions. These results indicated BM-DFAT possess the promoting effects of engraftment of human UCB.
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