Targeting continuous inflammation in pancreatic cancer microenvironment

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790674
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Tohoku University
• Principal Investigator: HAMADA Shin 東北大学, 医学(系)研究科(研究院), 助教 (20451560)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: microRNA / アポトーシス / 炎症性シグナル

Research Abstract

We assessed the effect of forced expression of miR-365, which was highly expressed in invasive pancreatic cancer compared with IPMN. miR-365 increased the cell viability of pancreatic cancer cells after the gemcitabine treatment, suggesting the induction of resistance. A comprehensive analysis of gene expression profiles in miR-365 introduced cells identified up-regulation of several NF kappa B target genes. Activation of NF kappa B pathway was confirmed by the increased expression of phosphorylated NF kappa B. On the other hand, miR-365 directly targeted apoptosis-related molecules such as SHC1 and BAX that led to the gemcitabine resistance.

Research Products

• [Journal Article] MiR-365 induces gemcitabine resistance in pancreatic cancer cells by targeting the adaptor protein SHC1 and pro-apoptotic regulator BAX (2014)
  • Author(s): Hamada S, Masamune A, Miura S, Satoh K, Shimosegawa T
  • Journal Title: Cell Signal Volume: 26(2) Pages: 179-185
  • DOI: 10.1016/j.cellsig.2013.11.003.
  • Peer Reviewed
• [Journal Article] miR-197 induces epithelial-mesenchymal transition in pancreatic cancer cells by targeting p120 catenin (2013)
  • Author(s): Hamada S, Satoh K, Miura S, Hirota M, Kanno A, Masamune A, Kikuta K, Kume K, Unno J, Egawa S, Motoi F, Unno M, Shimosegawa T
  • Journal Title: J Cell Physiol Volume: 228(6) Pages: 1255-1263
  • DOI: 10.1002/jcp.24280.
  • Peer Reviewed
• [Presentation] MiR-365によるゲムシタビン耐性誘導機構の検討 (2013)
  • Author(s): 濱田晋,佐藤賢一,正宗淳,菅野敦,菊田和宏,粂潔,廣田衛久,下瀬川徹
  • Organizer: JDDW
  • Place of Presentation: 東京
  • Year and Date: 2013-10-09
• [Presentation] MiR-365 indirectly regulates drug resistance-related molecule Aryl hydrocarbon receptor nuclear translocator expression in pancreatic ductal adenocarcinoma cells (2013)
  • Author(s): Hamada S, Satoh K, Masamune A, Kanno A, Kikuta K, Kume K, Unno J, Hirota M and Shimosegawa T
  • Organizer: DDW2013
  • Place of Presentation: Orlando USA
  • Year and Date: 2013-05-19
• [Presentation] Identification of inflammatory signal-stimulating microRNA in pancreatic duct cell carcinoma (2013)
  • Author(s): Hamada S, Satoh K, Masamune A, and Shimosegawa T
  • Organizer: JSGE-ITC2013
  • Place of Presentation: 鹿児島
  • Year and Date: 2013-03-23
• [Presentation] Identification of invasion-related microRNAs in pancreatic cancer by comprehensive analysis (2012)
  • Author(s): Hamada S, Satoh K, Hirota M, Kanno A, Masamune A, Kikuta K, Kume K, Unno J and Shimosegawa T
  • Organizer: UEGW 2012
  • Place of Presentation: Amsterdam Netherlands
  • Year and Date: 2012-10-23