Contribution of TET1 to malignant characteristics in digestive cancer cells.

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790682
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: The University of Tokyo
• Principal Investigator: KUDO Yotaro 東京大学, 医学部附属病院, 特任臨床医 (90608358)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 肝臓学

Research Abstract

Ten-Eleven-Translocation (TET) proteins mediate the conversion from 5-methylcytosine to 5-hydroxymethylcytosine (5-hmC), which is an intermediate of DNA demethylation. We aim to elucidate whether TET-mediated DNA demethylation is involved in biological properties in solid cancers. We have reported a loss of global 5-hmC level in a broad spectrum of human tumors. These findings suggest critical roles of abnormal DNA demethylation for oncogenic processes in solid tissues. Next, we focus on the role of TET1 protein in cancers, especially gastrointestinal and hepatic cancers. We found that TET1-depletion suppressed cell proliferation in various cell lines, and gene expression array analysis was carried out to validate responsible genes.

Research Products

• [Journal Article] Loss of 5-hydroxymethylcytosine is accompanied with malignant cellular transformation (2012)
  • Author(s): Kudo Y, Tateishi K, Yamamoto K, Yamamoto S, Asaoka Y, Ijichi H, Nagae G, Yoshida H, Aburatani H, Koike K
  • Journal Title: Cancer Science Volume: 103巻 Pages: 670-676
  • DOI: 10.1111/j.1349-7006.2012.02213.x.
  • Peer Reviewed
• [Presentation] Contribution of TET1 to malignant characteristics in digestive cancer cells (2013)
  • Author(s): Yotaro Kudo, Keisuke Tateishi, Tsuneo Ikenoue, Keisuke Yamamoto, Shinzo Yamamoto, Yoshinari Asaoka, Hideaki Ijichi, Genta Nagae, Masato Yonezawa, Motoaki Seki, Kiyokazu Shirai, Haruhiko Yoshida, Hiroyuki Aburatani, Kazuhiko Koike
  • Organizer: Cell Symposia: Cancer Epigenomics
  • Place of Presentation: Sitges, Spain
  • Year and Date: 20131006-08
• [Presentation] 悪性形質転換における DNA脱メチル化反応中間体ヒドロキシメチル化シトシンの検討 (2013)
  • Author(s): 工藤洋太郎、立石敬介、山本恵介、山本信三、浅岡良成、伊地知秀明、池上恒雄、白井聖一、永江玄太、吉田晴彦、油谷浩幸、小池和彦
  • Organizer: 第50回日本臨床分子医学会学術総会
  • Place of Presentation: 東京国際フォーラム, 東京
  • Year and Date: 20130412-13
• [Presentation] 消化器がんにおけるヒドロキシメチル化シトシンおよび DNA脱メチル化酵素 TET1の役割の検討 (2012)
  • Author(s): 工藤洋太郎、立石敬介、山本恵介、山本信三、浅岡良成、伊地知秀明、池上恒雄、白井聖一、永江玄太、吉田晴彦、油谷浩幸、小池和彦
  • Organizer: 第20回浜名湖シンポジウム
  • Place of Presentation: アクトシティ浜松コングレスセンター, 浜松
  • Year and Date: 20121222-23
• [Presentation] Loss of 5-hydroxymethylcytosine is accompanied with malignant cellular transformation (2012)
  • Author(s): Yotaro Kudo, Keisuke Tateishi, Yoshinari Asaoka, Hideaki Ijichi, Genta Nagae, Hiroyuki Aburatani, Kazuhiko Koike
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ニューオータニイン札幌, 札幌
  • Year and Date: 20120919-21
• [Presentation] 悪性形質転換におけるヒドロキシメチル化シトシンの低下 (2012)
  • Author(s): 工藤洋太郎、立石敬介、山本恵介、山本信三、浅岡良成、伊地知秀明、永江玄太、油谷浩幸、小池和彦
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 学術総合センター, 東京
  • Year and Date: 20120514-15