2014 Fiscal Year Final Research Report
Exploration of tumor suppressive factors of human pancreatic cancer by microRNA functional screening assay
| Project/Area Number |
24790683
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 膵がん / マイクロRNA / 機能スクリーニング |
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| Outline of Final Research Achievements |
The purpose of this study is to identify tumor-suppressive microRNAs (miRNA) of pancreatic cancer by functional screening assay, a phenotype-oriented screening method, and their functional analysis, which may lead to a new strategy of the treatment of pancreatic cancer. Microarray was designed to detect each clone contained in the virus library which expresses microRNA precursors. MicroRNAs that suppress proliferation of pancreatic cancer were identified by semi-quantitative comparison of each clone before and after introduction of miRNA library either in vitro or in vivo. Among these miRNAs was microRNA-34 (miR-34): one of the mechanisms of the tumor-suppressive action appears to be through cell cycle pathway, which was shown by gene expression analysis of pancreatic cell line transfected with miR-34.
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| Free Research Field |
消化器がん
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