2014 Fiscal Year Final Research Report
Functional analysis of Lgr5-positive epithelial stem cells in the process of intestinal epithelium regeneration and inflammation carcinogenesis.
| Project/Area Number |
24790697
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Shimane University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | GERD / TRP |
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| Outline of Final Research Achievements |
We have created the inflammation carcinogenesis model. As a method, 6-8-week-old male mice were injected with Azoxymethane (10 mg / kg) in an intraperitoneal manner, followed by a single cycle of Dextran Sulfate Sodium Salt (2.0%, 7 days) in drinking water and euthanized at 18 weeks. The study was done to evaluate by measuring the number and size of the tumor in colon. Mice were dissected, and we observed large intestine by stereomicroscope and histology. Using this model, we were able to study apoptosis, regeneration and growth mechanism of epithelial cells in inflammatory carcinogenesis processes. However, we did not lead to elucidate Lgr5 positive cells whether to act as a cancer stem cell in inflammatory carcinogenesis process. We think requires further study.
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| Free Research Field |
医歯薬学
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