2014 Fiscal Year Final Research Report
Factors that inhibit hepatocyte lipoapoptosis
Project/Area Number |
24790709
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Gastroenterology
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Research Institution | Nagasaki University |
Principal Investigator |
AKAZAWA Yuko 長崎大学, 原爆後障害医療研究所, 助教 (80582113)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | 脂肪酸 / アポトーシス |
Outline of Final Research Achievements |
Elevated serum free fatty acids and hepatocyte apoptosis are part of the features of nonalcoholic steatohepatitis (NASH). Cellular inhibitor of apoptosis (cIAP)-1 protein is an inhibitor of death receptor -mediated apoptosis. Aim of our study was to determine the role of cIAP-1 degradation during free fatty acid mediatedhepatocyte apoptosis.bcIAP-1 protein underwent degradation following treatment with palmitate in cultured HCC cells. SMAC mimetic JP1584, which induces rapid degradation of cIAP-1, significantly enhanced PA- mediated apoptosis in Mouse primary hepatocytes. However, JP 1584 did not sensitize primary hepatocytes from DR -/- mouse to apoptosis. These results indicated that degradation of cIAP-1 by PA enhances death receptor mediated pathway of lipoapoptosis. We are currently investigating the role of Troxerutin, a flavonoid, in FFA-mediated apoptosis. Troxerutin attenuated PA-mediated lipoapoptosis. We are currently investigating the mechanism that leads to this effect.
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Free Research Field |
消化器内科学
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