2013 Fiscal Year Final Research Report
Functional analysis and pharmacological testing of iPSc-derived cardiomyocyte as model for cardiac arrhythmia
Project/Area Number |
24790745
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Circulatory organs internal medicine
|
Research Institution | University of Toyama |
Principal Investigator |
KINOSHITA Koshi 富山大学, 医学薬学研究部(医学), 助教 (10585920)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Keywords | 不整脈 / iPS細胞 / QT延長 / 心筋誘導 / 薬剤応答性 / 疾患モデル |
Research Abstract |
We succeeded in inducing cardiomyocyte from iPS cells using only two chemicals, CHIR99021 and IWP-4. Beating was detected within ten days, similar to the conventional method using Activin A and BMP4 proteins. RT-PCR and immunostaining revealed that the induced cardiomyocyte expressed cardiomyocyte-specific genes or proteins. The alterations of beat rate and field potential duration in response to the adrenergic agonist were comparable between the cardiomyocyte derived from a patient carrying a mutation in KCNQ1 gene and healthy control. Using iPS cell-derived cardiomyocyte, various pharmacological testing will become available in the future.
|
-
[Journal Article] A590T mutation in KCNQ1 C-terminal helix D decreases IKs channel trafficking and function but not Yotiao interaction2014
Author(s)
Kinoshita K, Komatsu T, Nishide K, Hata Y, Hisajima N, Takahashi H, Kimoto K, Aonuma K, Tsushima E, Tabata T, Yoshida T, Mori H, Nishida K, Yamaguchi Y, Ichida F, Fukurotani K, Inoue H, Nishida N
-
Journal Title
J Mol Cell Cardiol
Volume: 72
Pages: 273-280
DOI
Peer Reviewed
-
[Journal Article] Identification and characterization of a novel genetic mutation with prolonged QT syndrome in an unexplained postoperative death2014
Author(s)
Hata Y, Mori H, Tanaka A, Fujita Y, Shimomura T, Tabata T, Kinoshita K, Yamaguchi Y, Ichida F, Kominato Y, Ikeda N, Nishida N.
-
Journal Title
Int J Legal Med
Volume: 128
Pages: 105-115
DOI
Peer Reviewed
-
[Journal Article] Characterization of a novel mutant KCNQ1 channel subunit lacking a large part of the C-terminal domain2013
Author(s)
Kimoto K, Kinoshita K, Yokoyama T, Hata Y, Komatsu T, Tsushima E, Nishide K, Yamaguchi Y, Mizumaki K, Tabata T, Inoue H, Nishida N, Fukurotani K
-
Journal Title
Biochem Biophys Res Commun
Volume: 440
Pages: 283-288
DOI
Peer Reviewed
-
[Journal Article] A novel missense mutation causing a G487R substitution in the S2-S3 loop of human ether-a-go-go-related gene channel2012
Author(s)
Kinoshita K, Yamaguchi Y, Nishide K, Kimoto K, Nonobe Y, Fujita A, Asano K, Tabata T, Mori H, Inoue H, Hata Y, Fukurotani K, Nishida N
-
Journal Title
J Cardiovasc Electrophysiol
Volume: 11
Pages: 1246-1253
DOI
Peer Reviewed
-
[Presentation] C-terminal helix D of KCNQ1 contributes to normal IKs channelfunction.2014
Author(s)
Takuto Komatsu, Koshi Kinoshita, Katsuya Kimoto, Yukiko Hata, Kei Aonuma, Eikichi Tsushima, Kohki Nishide, Nozomi Hisajima, Hiroyuki Takahashi, Kenkichi Fukurotani, Naoki Nishida, Toshihide Tabata
Organizer
第91回日本生理学会大会
Place of Presentation
鹿児島
Year and Date
2014-03-17
-
[Presentation] Functional Characterization of KCNQ1 Channel Subunit with an A590T mutation.2013
Author(s)
Koshi Kinoshita, Takuto Komatsu, Katsuya Kimoto, Kohki Nishide, Toshihide Tabata, Fukiko Ichida, Yoshiaki Yamaguchi, Kunihiro Nishida, Hiroshi Inoue, Yukiko Hata, Naoki Nishida
Organizer
Basic Cardiovascular Sciences (BCVS)
Place of Presentation
Las Vegas
Year and Date
2013-07-22
-
[Presentation] Abnormal modulation of hERG and KCNQ1 channels.2013
Author(s)
Kohki Nishide, Mario Kato, Yoshiaki Yamaguchi, Koshi Kinoshita, Akira Fujita, Yuki Nonobe, Yukiko Hata, Koichi Mizumaki, Hiroshi Inoue, Naoki Nishida, Toshihide Tabata
Organizer
第90回日本生理学会大会
Place of Presentation
東京
Year and Date
2013-03-27
-
[Presentation] Characterization of a New Mutant KCNQ1 Channel Subunit with aC-TerminalTruncation.2013
Author(s)
Katsuya Kimoto, Kohki Nishide, Koshi Kinoshita, Yuki Nonobe, Akira Fujita, Yoshiaki Yamaguchi, Yukiko Hata, Koichi Mizumaki, Hiroshi Inoue, Naoki Nishida, Toshihide Tabata
Organizer
第90回日本生理学会大会
Place of Presentation
東京
Year and Date
2013-03-27
-
-