2013 Fiscal Year Final Research Report
Novel 3D-culture System for iPS Cell-Derived Cardiomyocytes for Cardiac Repair
| Project/Area Number |
24790750
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Shinshu University |
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Principal Investigator |
SHIBA Yuji 信州大学, 医学部, 助教 (70613503)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | iPS細胞 |
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| Research Abstract |
Although pluripotent stem cell-derived cardiomyocytes are a promising tool for cardiac repair following cardiac injury, more study is required to improve the cell engraftment. We tested our novel 3D-culture system for the transplantation of stem cell-derived cardiomyocytes for not only graft cell survival but also electrical integration.
The 3D-culured human iPSC-cardiomyocytes survived following transplantation in injured guinea pig hearts, but they did not couple with host cardiomyocytes by fluorescent Ca sensor GCaMP3 imaging system. Additional study is needed to improve the host-graft electrical integration.
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[Journal Article] Crucial ro ole of hyaluronan in neointim mal formation after vascular in njury2013
Author(s)
Kashima Y, , Takahashi M M, Shiba Y, Ita ano N, Izawa A, Ko oyama J, Nakaayama J, Taniiguchi S, Kimata K, Ikeda U
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Journal Title
PLoS One
Volume: 8(3)
Pages: e558760
DOI
Peer Reviewed
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[Journal Article] Low levels of high-density lipoprotein cholesterol predict the presence of coronary artery disease in patients with aortic aneurysms.2013
Author(s)
Saigusa T, Izawa A, Miura T, Ebisawa S, Shiba Y, Miyashita Y, Tomita T, Koyama J, Fukui D, Takano T, Amano J, Ikeda U
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Journal Title
Angiology
Volume: (in press)
Peer Reviewed
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[Journal Article] hESC-derived cardiomyocytes electrically couple and suppress arrhythmias in injured hearts2012
Author(s)
Shiba Y, Fernandes S, Zhu WZ, Filice D, Muskheli V, Kim J, Palpant NJ, Gantz J, Moyes KW, Reinecke H, Van Biber B, Dardas T, Mignone JL, Izawa A, Hanna R, Viswanathan M, Gold JD, Kotlikoff MI, Sarvazyan N, Kay MW, Murry CE, Laflamme MA
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Journal Title
Nature
Volume: 489(7415)
Pages: 322-325
DOI
Peer Reviewed
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[Journal Article] Creation of human cardiac cell sheets using pluripotent stem cells2012
Author(s)
Matsuura K, Wada M, Shimizu T, Haraguchi Y, Sato F, Sugiyama K, Konishi K, Shiba Y, Ichikawa H, Tachibana A, Ikeda U, Yamato M, Hagiwara N, Okano T
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Journal Title
Biochem Biophys Res Commun
Volume: 425(2)
Pages: 321-327
DOI
Peer Reviewed
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