Epigenetic regulation induces the functional cardiomyocytes derived from human pluripotent stem cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790755
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Circulatory organs internal medicine
• Research Institution: Kyoto University
• Principal Investigator: OTSUJI Tomomi G. 京都大学, 物質-細胞統合システム拠点, 研究員 (50564754)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 移植・再生医療 / ヒトES/iPS細胞 / 心筋分化 / 心機能 / 機能細胞 / 大量培養法

Outline of Final Research Achievements

The cardiomyocytes (CMs) derived from Human ESC and iPSC (hESC/iPSC) are currently viewed as promising tools in regenerative medicine and pharmacological development. However, they are functionally heterogeneous, display insufficient biological efficacy and generally possess the electrophysiological properties seen in fetal CMs. Here, treating with a histone deacetylase inhibitor (HDACi) facilitates cardiac function in primitive hESC/hiPSC-CMs. HDACi-treated hESC/hiPSC-CM colonies showed appropriate responses to particular concentrations of known potassium ion channel inhibitors. In addition, we developed 3D sphere culture system without mechanical stirring for hESC/hiPSCs. Combination of these system may be optimized toward translation into a large-scale hESC/iPSC-CMs production format.

Free Research Field

幹細胞生物学