2015 Fiscal Year Final Research Report
CADM1 as a potential therapeutic target in small cell lung cancer
| Project/Area Number |
24790802
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 小細胞肺癌 / CADM1 / スプライシングバリアント / 分子標的治療 |
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| Outline of Final Research Achievements |
We investigate the roles of CADM1, a member of the immunoglobulin superfamily cell adhesion molecules, in small cell lung cancer (SCLC). Western blotting and RT-PCR analyses have revealed that CADM1 is significantly expressed in 11 of 14 SCLC cells growing in suspension cultures but in neither of 2 SCLC cells showing attached growth to plastic dishes, suggesting that CADM1 is involved in anchorage-independent growth in SCLC. Then, we demonstrate that SCLC expresses a unique splicing variant of CADM1. This variant is almost exclusively observed in SCLC and testis. Interestingly, suppression of CADM1 expression by shRNA reduced spheroid-like cell aggregation of SCLC cells. Immunohistochemistry demonstrates that CADM1 is strongly expressed in 24 of 34 (71%) SCLC. Overexpression of CADM1 was significantly associated with poor prognosis in surgical patients. These findings suggest that CADM1 enhances the malignant features of SCLC, and could provide a molecular target specific to SCLC.
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| Free Research Field |
肺癌
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