Differentiation from mouse ES cells to GH, LH and FSH producing endocrine cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790955
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: Nagoya University
• Principal Investigator: SUGA Hidetaka 名古屋大学, 医学部附属病院, 病院助教 (20569818)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 細胞分化 / 口腔外胚葉 / ラトケ嚢

Outline of Final Research Achievements

It has been reported that the oral ectoderm differentiates into anterior pituitary, tooth enamel, nasal epithelium and oral epithelium. In our previous report, we showed the the oral ectoderm differentiation method from mouse ES cells, but the differentiation efficiency was still low. In this study, we succeeded in oral ectoderm induction at high rate by the addition of component X. Moreover, with component Y treatment, mouse ES-derived oral ectoderm differentiates into Rathke's pouch. We established robust differentiation method from mouse ES cells into oral ectoderm, regardless of the lot difference of FBS or KSR.

Free Research Field

医歯薬学