2013 Fiscal Year Final Research Report
Development of leukemia stem cell-specific therapy based on establishment of induced pluripotent stem cells derived from acute myeloid leukemia.
| Project/Area Number |
24790964
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YOSHIMI Akihide 東京大学, 医学部附属病院, 特任助教 (80609016)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | iPS細胞 / 急性骨髄性白血病 / 慢性骨髄単球性白血病 / 標的遺伝子 / 発現解析 / エピゲノム解析 |
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| Research Abstract |
We tried various strategies using cell sorting , knockdown of p53, administration of some regulators on cell differentiation, and inhibitors of epigenetic regulators to establish acute myeloid leukemia-derived induced plurirpotent stem (iPS) cells. However, these trials resulted in failure. On the other hand, we succeeded in establishing chronic myelomonocytic leukemia (CMMoL)-patients derived iPS (CMMoL-iPS) cells. These CMMoL-iPS cells and hematopoietic cells which were differentiated from CMMoL-iPS cells showed higher proliferation rate compared with normal control cells. In addition, we identified some target genes of CMMoL by analyzing epigenetic and transcriptional profiles of these cells.
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[Journal Article] The shortest isoform of C/EBP・, Liver inhibitory protein (LIP), collaborates with Evi1 to induce AML in a mouse BMT model2013
Author(s)
Watanabe-Okochi N, Yoshimi A, Sato T, Ikeda T, Kumano K, Taoka K, Satoh Y, Shinohara A, Tsuruta T, Masuda A, Yokota H, Yatomi Y, Takahashi K, Kitaura J, Kitamura T, Kurokawa M
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Journal Title
Blood
Volume: 121
Pages: 4142-55
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[Journal Article] The shortest isoform of C/EBP・・ Liver inhibitory protein (LIP), collaborates with Evi1 to induce AML in a mouse BMT model2013
Author(s)
Watanabe-Okochi N, Yoshimi A, Sato T, Ikeda T, Kumano K, Taoka K, Satoh Y, Shinohara A, Tsuruta T, Masuda A, Yokota H, Yatomi Y, Takahashi K, Kitaura J, Kitamura T, Kurokawa M
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Journal Title
Rinsho Ketsueki
Volume: 54
Pages: 749-58
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[Journal Article] Evi1 Defines Leukemia-initiating Capacity and Tyrosine Kinase Inhibitor Resistance in Chronic Myeloid Leukemia
Author(s)
Sato T, Goyama S, Kataoka K, Nasu R, Tsuruta-Kishino T, Kagoya Y, Nukina A, Kumagai K, Kubota N, Nakagawa M, Arai S, Yoshimi A, Honda H, Kadowaki T, Kurokawa M
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Journal Title
Oncogene
Volume: (in press)
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[Journal Article] EVI1 oncogene promotes KRAS pathway through suppression of microRNA-96 in pancreatic carcinogenesis
Author(s)
Tanaka M, Suzuki HI, Shibahara J, Kunita A, Isagawa T, Yoshimi A, Kurokawa M, Miyazono K, Aburatani H, Ishikawa S, Fukayama M
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Journal Title
Oncogene
Volume: (in press)
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[Presentation] Recurrent CDC25C mutations drive malignant transformation in FPD/AML2014
Author(s)
Akihide Yoshimi, Takashi Toya, Masahito Kawazu, Toshihide Ueno, Ayato Tsukamoto, Hiromitsu Iizuka, Masahiro Nakagawa, Yasuhito Nannya, Shunya Arai, Motoshi Ichikawa, Hironori Harada, Kensuke Usuki, Yasuhide Hayashi, Etsuro Ito, Keita Kirito, Hideaki Nakajima, Hiroyuki Mano, Mineo Kurokawa
Organizer
American Association for Cancer Research 2014
Place of Presentation
San Diego, USA
Year and Date
20140405-09
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[Presentation] Evi1 Defines Leukemia-initiating Capacity and Tyrosine Kinase Inhibitor Resistance in Chronic Myeloid Leukemia2014
Author(s)
Tomohiko Sato, Susumu Goyama, Keisuke Kataoka, Ryo Nasu, Takako Tsuruta-Kishino, Yuki Kagoya, Arika Nukina, Katsuyoshi Kumagai, Naoto Kubota, Masahiro Nakagawa, Shunya Arai, Akihide Yoshimi, Hiroaki Honda, Takashi Kadowaki and Mineo Kurokawa
Organizer
AACR 2014
Place of Presentation
San Diego, USA
Year and Date
20140405-09
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[Presentation] The Genetic Landscape Of FPD/AML Revealed CDC25C Mutation As a Driver That Promotes Malignant Transformation2013
Author(s)
Akihide Yoshimi, Takashi Toya, Masahiro Nakagawa, Masahito Kawazu, Yasuhito Nannya, Motoshi Ichikawa, Shunya Arai, Hironori Harada, Kensuke Usuki, Yasuhide Hayashi, Etsuro Ito, Keita Kirito, Hideaki Nakajima, Hiroyuki Mano and Mineo Kurokawa
Organizer
55th ASH Annual Meeting and Exposition
Place of Presentation
New Orleans, USA
Year and Date
20131207-10
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[Presentation] Clonal and mutational evolution reveals genetic mechanisms of leukemia transformation of FPD/AML2013
Author(s)
吉見 昭秀, 遠矢 嵩, 飯塚 浩光, 荒井 俊也, 中川 正宏, 河津 正人, 市川 幹, 桐戸 敬太, 間野 博行, 黒川 峰夫
Organizer
第76回日本血液学会学術集会
Place of Presentation
Sapporo, Japan
Year and Date
20131011-13
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[Presentation] Exome sequencing combined with single cell sequencing reveals genetic mechanisms of malignant transformation in FPD/AML2013
Author(s)
Akihide Yoshimi, Takashi Toya, Hiromitsu Iizuka, Shunya Arai, Masahiro Nakagawa, Masahito Kawazu, Motoshi Ichikawa, KeitaKirito, Hiroyuki Mano, Mineo Kurokawa
Organizer
第72回日本癌学会総会
Place of Presentation
Yokohama, Japan
Year and Date
20131003-05
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[Presentation] Pathological analysis of familial platelet disorder with propensity to myeloid malignancy2013
Author(s)
Akihide Yoshimi, Hiromitsu Iizuka, Takashi Toya, Tomohiko Sato, Masahito Kawazu, Keiki Kumano, Motoshi Ichikawa, Keita Kirito, Hiroyuki Mano, Shunya Arai, Mineo Kurokawa
Organizer
Runx Workshop 2013
Place of Presentation
Wilsede, Germany
Year and Date
20130616-19
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[Presentation] Evi1はポリコーム複合体との相互作用を介してエピジェネティックにPTEN の発現を抑制し, AKT/mTOR シグナルを活性化する2012
Author(s)
吉見昭秀, 合山進, 渡辺-大河内直子, 吉識由美子, 南谷泰仁, 仁田英里子, 荒井俊也, 佐藤智彦, 島辺宗健, 中川正宏, 今井陽一, 北村俊雄, 黒川峰夫
Organizer
第8回血液学若手研究者勉強会(麒麟塾)
Place of Presentation
品川
Year and Date
2012-07-07
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