2013 Fiscal Year Final Research Report

Screening of a novel factor that regulates hematopoiesis using a homozygous mutant ES cell library

Research Project

Project/Area Number	24790972
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Hematology
Research Institution	Osaka University
Principal Investigator	TOKUNAGA MASAHIRO 大阪大学, 医学(系)研究科(研究院), 助教 (90597410)
Research Collaborator	HORIE Kyoji 奈良県立医科大学, 医学部, 教授 (30333446) YAMANISHI Ayako 大阪大学, 医学系研究科, 特任研究員
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	血球分化 / ES細胞 / スクリーニング / ノックアウトマウス
Research Abstract	In this study, to screen for a mutant whose hematopoietic differentiation is disturbed, we co-cultured several homozygous mutant ES clones with OP9, a stromal cell line supporting hematopoietic differentiation. As a result, we found out that in one mutant clone, this differentiation was severely disturbed. The responsible gene of this mutant is a novel, uncharacterized gene (unpublished data). Then, we generated a knockout mouse of this gene. Intriguingly, null mutant mice were embryonic lethal by E6.5, demonstrating that this gene plays essential roles in early development. Further investigation of this gene may give a new insight in hematopoietic development.

(1 results)