Role of calcium/calmodulin kinase IV on podocyte function in lupus nephritis

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790999
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Nagasaki University
• Principal Investigator: ICHINOSE Kunihiro 長崎大学, 医歯(薬)学総合研究科, 助教 (60437895)
• Co-Investigator(Kenkyū-buntansha): KAWAKAMI Astushi 長崎大学, 大学院・医歯薬学総合研究科展開医療科学講座(第一内科), 教授 (90325639) ; NAKAMURA Hideki 長崎大学, 病院第一内科, 講師 (10437832) ; IWAMOTO Naoki 長崎大学, 病院第一内科, 助教 (80437897)
• Co-Investigator(Renkei-kenkyūsha): KOGA Tomohiro 長崎大学, 病院第一内科, 助教 (90537284)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 全身性エリテマトーデス / ループス腎炎 / ポドサイト / CaMKIV

Research Abstract

T cell from patients with systemic lupus erythematosus display increased expression of calcium/calmodulin kinase IV (CaMKIV). CaMKIV expression was found increased in podocytes of Lupus nephritis(LN) kidney biopsy specimens. The levels of nephrin and CaMKIV correlated in an inverse manner. Interestingly, culture of AB8/13 podocytes in the presence of IgG from LN sera led to 1.5-fold increase in the expression of CaMKIV mRNA. Gene array analysis revealed that the expression of genes related to the cell activation including CaMKIV increased significantly (P<0.05) and the regulation of neural precursor cell proliferation decreased significantly (P<0.01) in podocytes treated with IgG from LN patients. Our data demonstrate increased expression of CaMKIV in LN podocytes which may represent the result of exposure to IgG. We suspect that increased CaMKIV expression may contribute to the inability of podocytes to maintain the integrity of glomerular basement membrane in LN patients.

Research Products

• [Presentation] ループス腎炎におけるCalcium/calmodulin dependent kinase protein type IV のポドサイト機能に対する影響(第2 報) (2014)
  • Author(s): 一瀬邦弘, 牛草健, 梅田雅孝, 中島好一, 鈴木貴久, 寶來吉朗, 岡田覚丈, 川㞍真也, 岩本直樹, 玉井慎美, 中村英樹, 折口智樹, 川上純
  • Organizer: 第58回日本リウマチ学会総会・学術集会
  • Place of Presentation: 東京・グランドプリンス新高輪本館及び国際館パミール
  • Year and Date: 20140425-27
• [Presentation] Role of calcium/calmodulin kinase IV on podocyte function in lupus nephritis (2013)
  • Author(s): Kunihiro Ichinose, Takeshi Ushigusa, Tomohiro Koga, George C.Tsokos, Atsushi Kawakami
  • Organizer: The ACR/ARHP 2013 Annual Meeting
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 20131025-30
• [Presentation] ループス腎炎におけるCalcium/calmodulin dependent kinase protein type IV のポドサイト機能に対する影響 (2013)
  • Author(s): 一瀬邦弘, 牛草健, 川上純
  • Organizer: 第56回日本腎臓学会学術総会
  • Place of Presentation: 東京・東京国際フォーラム
  • Year and Date: 20130510-12
• [Presentation] Role of calcium/calmodulin kinase IV on podocyte function in lupus nephritis (2013)
  • Author(s): Kunihiro Ichinose, Takeshi Ushigusa, Tomohiro Koga, George C.Tsokos, Atsushi Kawakami
  • Organizer: Immunology 2013
  • Place of Presentation: Honolulu, HI, USA
  • Year and Date: 20130503-07
• [Presentation] ループス腎炎におけるCalcium/calmodulin dependent kinase protein type IV のポドサイト機能に対する影響 (2013)
  • Author(s): 一瀬邦弘, 牛草健, 梅田雅孝, 中島好一, 鈴木貴久, 寶來吉朗, 岡田覚丈, 川㞍真也, 岩本直樹, 玉井慎美, 中村英樹, 折口智樹, 川上純
  • Organizer: 第57回日本リウマチ学会総会・学術集会
  • Place of Presentation: 京都・国立京都国際会館
  • Year and Date: 20130418-20