2013 Fiscal Year Final Research Report
Analysis of resistance mechanisms and epidemiology of beta-lactamase inhibitor-resistant gram-negative bacillus
| Project/Area Number |
24791020
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Infectious disease medicine
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| Research Institution | Nagoya University |
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Principal Investigator |
KIMURA Kouji 名古屋大学, 医学(系)研究科(研究院), 講師 (50425675)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | Klebsiella oxytoca / ベータラクタマーゼ阻害剤耐性 / ベータラクタマーゼRbiA |
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| Research Abstract |
We analyzed clinical isolates of beta-lactamase inhibitor-resistant Klebsiella oxytoca, which suspected to spread nosocomially. The pulsed-field gel electrophoresis (PFGE) analysis showed that these clinical isolates have identical genetical background. The electrophoresis of isoelectric point suggested that the clinical isolate produced one kind of beta-lactamase. Moreover, we revealed that these clinical isolates produced a beta-lactamase RbiA, which we reported previously. We generated the E. coli producing a beta-lactamase RbiA by transformation. The E. coli showed beta-lactamase inhibitor-resistance, suggesting that producing a beta-lactamase RbiA is the major cause of beta-lactamase inhibitor-resistance in the clinical isolates of beta-lactamase inhibitor-resistant Klebsiella oxytoca.
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[Presentation] 連鎖球菌感染症にみられる新しい知見2012
Author(s)
木村幸司, 長野則之, 長野由紀子, 外山雅美, 荒川宜親
Organizer
第61回日本感染症学会東日本地方会学術集会, 第59回日本化学療法学会東日本支部総会, 合同学会
Place of Presentation
東京 ホテル日航東京
Year and Date
20121010-12
Invited
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