2013 Fiscal Year Final Research Report
Development of novel disease modeling for L-asparaginage associated acute pancreatitis
| Project/Area Number |
24791051
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | 独立行政法人国立病院機構長良医療センター(臨床研究部) (2013)
Gifu University (2012) |
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Principal Investigator |
FUNATO Michinori 独立行政法人国立病院機構長良医療センター(臨床研究部), その他部局等, その他 (30420350)
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| Research Collaborator |
OSAFUNE Kenji 京都大学, iPS細胞研究所, 准教授 (80502947)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 急性膵炎 / iPS細胞 / 分化誘導法 |
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| Research Abstract |
L-asparaginase-associated pancreatitis is occasionally life-limiting, thus cell-based assay may be helpful to understand the mechanism of the important problem. Currently, we established iPS cells from two females with a history of L-asparaginase-associated pancreatitis. In addition, we modified previously reported differentiation protocol to pancreatic lineage on the basis of developmental biology insight, and have succeeded in the efficient generation of pancreatic progenitor cells. Furthermore, We have performed high-throughput screening of chemical compounds and identified a small molecule to efficiently induce pancreatic progenitor cells into pancreatic exocrine lineages. The small molecule expands differentiated pancreatic exocrine cells, and acts synergistically with indolactam V.
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[Presentation] 病態モデル作製に向けたヒトiPS/ES細胞から膵外分泌細胞への高効率分化誘導法の開発2014
Author(s)
舩戸道徳, 豊田太郎, 近藤恭士, 細川吉弥, 須藤智美, 沖田圭介, 浅香勲, 上杉志成, 加藤善一郎, 太田章, 山中伸弥, 近藤直実, 長船健二
Organizer
日本再生医療学会総会(第13回)
Place of Presentation
京都
Year and Date
2014-03-06
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[Presentation] Development of an efficient differentiation method from iPSCs/ESCs into pancreatic exocrine lineages towards novel pancreatic disease models2013
Author(s)
Michinori Funato, Taro Toyoda, Yasushi Kondo, Yoshiya Hosokawa, Tomomi Sudo, Keisuke Okita, Isao Asaka, Motonari Uesugi, Zen-Ichiro Kato, Akira Ohta, Shinya Yamanaka, Naomi Kondo, Kenji Osafune
Organizer
ISSCR 11th Annual Meeting
Place of Presentation
Boston, USA
Year and Date
20130612-15
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[Presentation] 病態解析に向けたヒトiPS/ES 細胞から膵外分泌細胞への高効率分化誘導法の開発2013
Author(s)
舩戸道徳, 豊田太郎, 近藤恭士, 細川吉弥, 須藤智美, 沖田圭介, 浅香勲, 上杉志成, 加藤善一郎, 太田章, 山中伸弥, 近藤直実, 長船健二
Organizer
日本再生医療学会総会(第12回)
Place of Presentation
横浜
Year and Date
2013-03-22
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