2015 Fiscal Year Final Research Report
Biochemical study for pathological mechanism of brain malformation with intractable epilepsy in childhood
| Project/Area Number |
24791081
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ITOH MASAYUKI 国立研究開発法人国立精神・神経医療研究センター, 神経医療研究センター・神経研究疾病研究第二部, 室長 (50243407)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 難治性てんかん / 限局性皮質異形成 / エピジェネティクス |
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| Outline of Final Research Achievements |
The prevalence of epilepsy is estimated as about 1.0%, of which 20% is intractable epilepsy in childhood. Among them, focal cortical dysplasia (FCD) is one of major diseases. Especially, FCD type IIb is well known to appear specific cells in the lesion. They are thought to have the both biological characters of neuron and astrocyte. However, we have never seen the evidence since 1970s, the first described those cells. In the present study, we investigated epigenetic mechanism and abnormal differentiation of pathological cells in FCD.
We performed immunohistochemistry with the antibodies of 5-methylcytosine and 5-methylcytidine. However, there is no different expression pattern of pathological cells. Next, we tried to make inducible formation of pathological cells, using GFAP-driven SH-SY5Y (neuroblastoma cell line). We made various vectors construct and selected some working vectors. We advance to establish vector-contained cells.
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| Free Research Field |
医歯薬学
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