Fecal microflora composition change in neonates with intensive theraphy

2014 Fiscal Year Final Research Report

• Project/Area Number: 24791120
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Kitasato University
• Principal Investigator: ITO Takashi 北里大学, 生命科学研究所, 研究員 (90383629)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 腸内細菌叢 / 新生児 / 早産児

Outline of Final Research Achievements

The composition of microflora may be influenced by various factors, such as nutrition and antibiotics treatment. The aim of this study is to understand the sequential change of microbial flora in the feces from preterm baby through 16S rRNA PCR assay. The fecal samples were obtained from 33 infants(31.6±0.5weeks gestation, 1398±83g birth body weight ). After bacterial DNA was extracted, subjected to PCR amplification. The result was that supplement of antibiotics did not influence the distribution of each bacterial species. In the group of probiotics treatment and the group of breast milk only, the colony of Bifidobacterium was found at very early postnatal day, and S.aureus was not colonized until the first week of life in the group of breast milk. In this study, probiotics and breast milk may work to compose the balanced microflora for preterm infants in NICU, long term observation is required to determine if this contribute to adverse outcome in future.

Free Research Field

新生児学