A development of easy-to-use method for diagnosis of scabies by DNA amplification and identification of drug-tolerant scabies for antiscabietic drug

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791131
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Hirosaki University
• Principal Investigator: MINAKAWA Satoko 弘前大学, 医学部附属病院, 医員 (20436033)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 疥癬 / ヒゼンダニ / 遺伝子

Research Abstract

We have to remember the scabies as highly infectious disease. The purpose of this study is a development of easy-to-use method for diagnosis of scabies by DNA amplification. The DNA extracted from scabies's bodies and eggs was used as a template. We researched by DNA sequencing of the polymerase chain reaction (PCR) products and Loop-Mediated Isothermal Amplification(LAMP). We examined the patients with highly pruritic red papules, their family and all healthcare professionals in charge of the patients after physical examination.

Research Products

• [Journal Article] Mutational analysis on 16 Japanese population cases with epidermolysis bullosasimplex (2013)
  • Author(s): Minakawa S, Nakano H, Nakajima K, Matsuzaki Y, Takiyoshi N, Akasaka E,Rokunohe D, Sawamura D
  • Journal Title: J Dermatol Sci Volume: 72(pii: S0923-1811(13)00275-2) Pages: 330-332
  • Peer Reviewed
• [Journal Article] Tegafur/gimeracil/oteracil (TS-1) induced StevenseJohnson syndrome:Case report (2013)
  • Author(s): Minakawa S, Matsuzaki Y, Nakajima K, Nakano H, Sawamura D
  • Journal Title: Dermatologica Sinica Volume: 31 Pages: 154-156.
  • DOI: 10.1016/j.dsi.2012.12.005
  • Peer Reviewed
• [Journal Article] EPR spectroscopic investigation of psoriatic finger nails (2013)
  • Author(s): Nakagawa K, Minakawa S, Sawamura D
  • Journal Title: Skin Res Technol Volume: 19 Pages: 450-3
  • DOI: 10.1111/srt.12068
  • Peer Reviewed
• [Journal Article] Q-switched ruby laser therapy and long-term follow-up evaluation of small to medium-sized congenital melanocyticnaevi (2012)
  • Author(s): Minakawa S, Takeda H, Korekawa A, Kaneko T, Urushidate, Sawamura D
  • Journal Title: Clin Exp Dermatol Volume: 37 Pages: 438-40
  • DOI: 10.1111/j.1365-2230.2011.04226.x
  • Peer Reviewed
• [Journal Article] Acantholysis caused repeated hemorrhagic bullae in a case of acantholytic acanthoma (2012)
  • Author(s): Minakawa S, Matsuzaki Y, Nakano H, Sawamura D, Elenitsas R
  • Journal Title: J Dermatol Volume: 39 Pages: 1107-8
  • DOI: 10.1111/j.1346-8138.2012.01564.x
  • Peer Reviewed
• [Journal Article] A Case of Erythema Nodosum Associated with Subareolar Abscess Journal of Cosmetics (2012)
  • Author(s): Minakawa S, Matsuzaki Y, Rokunoke D, Akasaka E, Nakajima K, Aizu T, Kaneko T, Nakano H, Takashima T, Sawamura D
  • Journal Title: Dermatological Sciences andApplications Volume: 2 Pages: 263-4
  • DOI: 10.4236/jcdsa.2012.24049
  • Peer Reviewed
• [Journal Article] Histologic features of nail cosmetics (2012)
  • Author(s): Anolik RB, Elenitsas R, Minakawa S, Nguyen J, Rubin AI
  • Journal Title: Am J Dermatopathol Volume: 34 Pages: 412-5
  • DOI: 10.1097/DAD.0b013e3182377005
  • Peer Reviewed
• [Journal Article] Spectroscopic evidence of abnormal structure of psoriasis vulgaris stratumcorneum. (2012)
  • Author(s): Nakagawa K, Minakawa S, Sawamura D
  • Journal Title: J Dermatol Sci Volume: 65 Pages: 222-4
  • DOI: 10.1016/j.jdermsci.2012.01.002
  • Peer Reviewed
• [Presentation] 単純型表皮水疱症 16例の遺伝子診断 (2013)
  • Author(s): 皆川智子,中野 創,中島康爾,澤村大輔,池田志斈,玉井克人,三橋善比古
  • Organizer: 第77回日本皮膚科学会東部支部学術大会
  • Place of Presentation: 大宮ソニックシティ(さいたま市)
  • Year and Date: 20130921-22
• [Presentation] 即時型インスリンアレルギーと考えられた1例 (2013)
  • Author(s): 皆川智子,金子高英,中野創,澤村大輔,森山貴子,森本武史
  • Organizer: 日本皮膚科学会青森地方会第364回例会
  • Place of Presentation: 弘前大学医学部コミュニケーションセンター(弘前市)
  • Year and Date: 2013-11-24
• [Presentation] 単純型表皮水疱症の1例 (2013)
  • Author(s): 皆川智子,中島康爾,中野創,澤村大輔,千葉純子
  • Organizer: 日本皮膚科学会青森地方会第363回例会
  • Place of Presentation: 八戸パークホテル(八戸市)
  • Year and Date: 2013-08-25
• [Presentation] 家族性良性慢世天疱瘡の1例 (2013)
  • Author(s): 皆川智子,松﨑康司,金子高英,中野創,澤村大輔,三橋善比古
  • Organizer: 第40回皮膚かたち研究会
  • Place of Presentation: 石垣記念ホール(東京)
  • Year and Date: 2013-07-28
• [Presentation] Stevens -Johnson syndrome associated with TS-1 (2013)
  • Author(s): Minakawa S, Matsuzaki Y, Nakajima K, Nakano H, Sawamura D
  • Organizer: 71th AAD Annual Meeting
  • Place of Presentation: Florida(America)
  • Year and Date: 2013-03-02
• [Presentation] ウステキヌマブで改善した乾癬の2例 (2012)
  • Author(s): 皆川智子,金子高英,松崎康司,中野創,澤村大輔,木村淳也
  • Organizer: 日本皮膚科学会青森地方会第359回例会
  • Place of Presentation: ホテルニューキャッスル(弘前市)
  • Year and Date: 2012-09-02
• [Presentation] 有棘細胞癌,Bowen病,日光角化症を伴った眼皮膚白皮症の1例 (2012)
  • Author(s): 皆川智子,金子高英,松﨑康司,中野創,澤村大輔,三上誠,鈴木民夫,三橋善比古
  • Organizer: 第39回皮膚かたち研究学会
  • Place of Presentation: 東京慈恵会医科大学(東京)
  • Year and Date: 2012-07-29