2013 Fiscal Year Final Research Report
Role of HMGB1 in skin reperfusion model
| Project/Area Number |
24791149
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Hamamatsu University School of Medicine |
|---|
Principal Investigator |
KASUYA Akira 浜松医科大学, 医学部附属病院, 助教 (60599421)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | 褥瘡 / 虚血再灌流 / マウスモデル / HMGB1 |
|---|
| Research Abstract |
High mobility group box-1 (HMGB1) is capable of activating macrophages in injured tissues during ischemia reperfusion (IR) injury. IR is mediated through tissue ischemia, leading to excessive inflammation and enhanced skin damage. We investigated the role of HMGB1 in the experimental IR injury model. The experimental IR injury induced HMGB1 release in the epidermal keratinocytes. Furthermore, edema was found in the dermis. To investigate the role of HMGB1 on edema, we initially quantified the vascular hyperpermeability in vivo administering fluorescence-labeled nanoparticles. Next, anti-HMGB1 neutralizing antibody was administered in mice 12 hours before IR. We found that blockade of HMGB1 suppressed vascular leakage after IR and accelerated mouse wound healing in the present model. These results indicate that HMGB1 plays a crucial role in the development of cutaneous IR injury, and may be a therapeutic target for pressure ulcer.
|
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Anti-HMGB1 antibody (αHMGB1) promotes wound healing by decreasing vascular hyperpermeability during ischemia-reperfusion (IR) injury2012
Author(s)
Akira Kasuya, Satoshi Hirakawa, Keyue Liu, Akihiro Kishimura, Yasutaka Anraku, Kazunori Kataoka, Mikako Ogawa, Shuji Mori, Hideo K. Takahashi, Jun-ichi Sakabe, Yasuko Tomono, Masahiro Nishibori, Yoshiki Tokura
Organizer
日本研究皮膚科学会 第37回年次学術大会・総会
Place of Presentation
那覇
Year and Date
2012-12-07
