Development of highly sensitive and rapid ELISA using luminometer and its application to detect autoantibodies in dermatomyositis

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791150
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Nagoya University
• Principal Investigator: SAWADA Masaki 名古屋大学, 医学部附属病院, 助教 (80467315)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 皮膚筋炎 / 自己抗体 / ELISA / リコンビナントタンパク

Research Abstract

Although recently identified dermatomyositis-associated autoantibodies are very useful in clinics,they can be identified by a very minor population of laboratory. Moreover, immunoprecipitation methods used in the laboratories cannot produce results in a short time. We have developed technically easy experimental methods using in vitro translation and transcription system to detect autoantibodies; the preparation of plasmids harboring cDNA in interest->expression of biotinylated recombinant protein by a commercial kit->captured ELISA with streptavidin-coated plates. This is an epoch-making method which can be constructed for approximate one week after getting a cDNA inserted into a vector with T7 promotor.

Research Products

• [Journal Article] Therapy-related myelodysplastic syndrome after alkylating agents and platinum-based therapy for malignant melanoma (2014)
  • Author(s): Nin-Asai R, Sawada M, Matsumoto T, Saito S, Yokota K, Akiyama M
  • Journal Title: J Dermatol Volume: 41 Pages: 178-9
• [Journal Article] Clinicopathologic analysis of 66 Japanese thin melanomas with metastasis of sentinel or regional lymph node (2013)
  • Author(s): Mori M, Sugiura M, Kono M, Matsumoto T, Sawada M, Yokota K, Yasue S, Shibata S, Sakakibara A, Nakamura S, Tomita Y, Akiyama M
  • Journal Title: J Cutan Pathol Volume: 40 Pages: 1027-34
• [Journal Article] Benefit of skin ultrasound examination in determining the area of inguinal lymph node dissection (2013)
  • Author(s): Kobayashi T, Yokota K, Sawada M, Matsumoto T, Akiyama M
  • Journal Title: J Dermatol Volume: 40 Pages: 765-6
• [Journal Article] Postoperative DAV-IFN-β therapy does not improve survival rates of stage II and stage III melanoma patients significantly (2013)
  • Author(s): Matsumoto T, Yokota K, Sawada M, Sakakibara A, Shibata S, Yasue S, Tomita Y, Yatsuya H, Akiyama M
  • Journal Title: J Eur Acad Dermatol Venereol Volume: 27 Pages: 1514-20
• [Journal Article] 大腸癌術後フォローCT にて発見された腹部脂肪織内非医原性伏針の1例 (2013)
  • Author(s): 小林 智子, 横田 憲二, 澤田 昌樹, 松本 高明, 秋山 真志
  • Journal Title: 皮膚科の臨床 Volume: 56 Pages: 102-105
• [Journal Article] 左膝蓋外側に発症した無色素性黒色腫の1例 (2012)
  • Author(s): 水谷 和広, 榊原 章浩, 横田 憲二, 澤田 昌樹, 松本 高明, 富田 靖, 星野 臣平
  • Journal Title: 皮膚科の臨床 Volume: 54 Pages: 930-931
• [Journal Article] Proposed classification of longitudinal melanonychia based on clinical and dermoscopic criteria
  • Author(s): Sawada M, Yokota K, Matsumoto T, Shibata S, Yasue S, Sakakibara A, Kono M, Akiyama M
  • Journal Title: Int J Dermatol Volume: (in press)
• [Presentation] 皮膚悪性黒色腫に対するセンチネルリンパ節生検施行後のリンパ流の変化について検討 (2013)
  • Author(s): 横田 憲二, 澤田 昌樹, 松本 高明, 秋山 真志
  • Organizer: 第29回日本皮膚悪性腫瘍学会学術大会
  • Place of Presentation: 甲府, 山梨県
  • Year and Date: 2013-08-09
• [Presentation] 臀部粉瘤より発生した有棘細胞癌の一例 (2013)
  • Author(s): 長谷川 佳恵, 横田 憲二, 河野 通浩, 澤田 昌樹, 松本 高明, 秋山 真志
  • Organizer: 第29回日本皮膚悪性腫瘍学会学術大会
  • Place of Presentation: 甲府, 山梨県
  • Year and Date: 2013-08-09
• [Presentation] Longitudinal melanonychia in children (2012)
  • Author(s): Sawada M, Yokota K, Matsumoto T, Shibata S, Yasue S, Sakakibara A, Kono M, Akiyama M
  • Organizer: 3rd World congress of dermoscopy
  • Place of Presentation: Brisbain, オーストラリア
  • Year and Date: 20120517-19
• [Presentation] 巨大脂腺上皮腫の1例 (2012)
  • Author(s): 澤田 昌樹, 横田 憲二, 松本 高明, 秋山 真志
  • Organizer: 第76回日本皮膚科学会東部支部学術大会
  • Place of Presentation: 札幌, 北海道
  • Year and Date: 2012-09-29
• [Presentation] メルケル細胞癌の3例 (2012)
  • Author(s): 澤田 昌樹, 杉浦 一充, 横田 憲二, 松本 高明, 武市 拓也, 長坂 徹郎, 片野晴隆, 秋山 真志
  • Organizer: 第111回日本皮膚科学会総会
  • Place of Presentation: 京都, 京都府
  • Year and Date: 2012-06-02