Development of therapeutic RNA interference of human melanoma for the purpose of database design

2014 Fiscal Year Final Research Report

• Project/Area Number: 24791168
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: NAKAI Noriaki 京都府立医科大学, 医学(系)研究科(研究院), 講師 (80453108)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: メラノーマ / 分子治療 / Mitf / ヒト

Outline of Final Research Achievements

We synthesized 6 types of CRISPR/Cas9-Mitf corresponding to the Mitf sequence which is involved in melanin synthesis as well as differentiation of melanocyte and melanoma, and transfected them into human melanoma cell lines. CRISPR/Cas9-Mitf-4-mediated knockout of Mitf suppressed viability of HMV-II melanoma by 13% in comparison with control HMV-II melanoma. Low expression of Mitf mRNA in control amelanotic human melanoma SK-Mel 28 and in control melanotic human melanoma G361, and high expression of Mitf mRNA in control melanotic human melanoma HMV-II were confirmed. CRISPR/Cas9-mediated knockout of Mitf might suppress growth of certain types of human melanoma. While Mitf mRNA expression levels in human melanoma might not be associated with melanin production ability.

Free Research Field

皮膚悪性腫瘍