2013 Fiscal Year Final Research Report
Analysis of filaggrin-null mice in dry environment to understand the pathogenic mechanism of atopic dermatitis
| Project/Area Number |
24791176
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 皮膚炎症・再生学 / アトピー性皮膚炎 |
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| Research Abstract |
Loss-of-function mutations in the filaggrin gene are major predisposing factors for atopic dermatitis. In this study, we hypothesized that dry environment might influence the impact of filaggrin deficiency and we evaluated filaggrin-null (Flg-/-) mice in dry condition. Dry condition exacerbated scaly skin gross phenotype in Flg-/- mice, which resembled Ichthyosis vulgaris, while skin in wild-type littermates were not apparently affected. Histological examination revealed acanthosis and large, thick scales focally in Flg-/- skin in dry condition, indicating abnormal cornification. Both of "inside-to-outside" and "outside-to-inside" permeation through Flg-/- stratum corneum were more enhanced in dry condition, as compared with wild-type stratum corneum. Thus, the skin phenotype and barrier disruption due to lack of filaggrin became more prominent in dry condition, indicating the importance of environmental factor in development of skin phenotype in filaggrin deficiency.
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