2014 Fiscal Year Final Research Report
Establishment of in vitro system to elucidate the mechanism underlying epileptogenesis in EL mice at an early neurodevelopmental stage and its therapeutic application
| Project/Area Number |
24791230
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Psychiatric science
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 胚性幹細胞（ES細胞） / ヒト側頭葉てんかん / 自然発症モデル / ELマウス / てんかん原性獲得 / 神経幹細胞 / 神経発達異常 |
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| Outline of Final Research Achievements |
The purpose of this project is to establish in vitro system to detect and recapitulate the unusual phenomena which could cause ictogenesis and epileptogenesis in EL mice at an early stage of neurodevelopment. To develop this system, EL-mice derived embryonic stem cells (EL-ESCs) were established. To analyze the processes of neural differentiation of EL-ESCs by modified NSS method which is a simple method to induce uni-directional differentiation of ESCs into neural stem cells (NSCs), EL-ESCs uni-directionally differentiated into NSCs as well as HK-ESCs which are derived from B57BL/6 mice as control. However, long term culture (at least 6 days under differentiation condition) promoted astrocytogenesis of EL-ESCs-derived NSCs, whereas NSCs derived from HK-ESCs mainly generating neurogenesis in this period. By contrast, NSCs established from both EL-ESCs and HK-ESCs normally proliferated under the proliferative condition.
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| Free Research Field |
幹細胞生物学
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