Development of a novel system measuring a circadian rhythm of erythrocytes

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791255
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Psychiatric science
• Research Institution: Keio University
• Principal Investigator: OHSAWA Yosuke 慶應義塾大学, 医学部, 助教 (50528429)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: ペルオキシレドキンシン / 概日リズム / 赤血球 / BRET

Research Abstract

I developed a measuring system based on bioluminescence resonance energy transfer (BRET). The system can sequentially monitor the homodimer formation of Peroxyredoxin 2 (PRX2). The temporal resolution of the luminescent data was dramatically improved by measuring the PRX2 dimeric rhythm at 10-minute intervals, which enabled us to calculate the acrophase or free-running period from the luminescent data with high accuracy.
This research suggested that hydrogen peroxide acts as an entraining agent (Zeitgeber) on the oxidation-reduction rhythm of PRX2. The elucidation of the time-keeping mechanism of the redox PRX2 rhythm in erythrocytes which lack a cell nucleus and clock genes may contribute greatly to our understanding of how the humoral or neuronal circadian signals control the peripheral clocks, and how the internal desynchronization between peripheral tissues occurs.

Research Products

• [Journal Article] In vitro circadian period is associated with circadian/sleep preference (2013)
  • Author(s): Hida A, Kitamura S, Ohsawa Y, Enomoto M, Katayose Y, Motomura Y, Moriguchi Y, Nozaki K, Watanabe M, Aritake S, Higuchi S, Kato M, Kamei Y, Yamazaki S, Goto Y, Ikeda M, Mishima K
  • Journal Title: Sci Rep Volume: 3, 2074 Pages: 1-7
  • DOI: 10.1038/srep02074
  • Peer Reviewed
• [Journal Article] Rhythmic expression of circadian clock genes in human leukocytes and beard hair follicle cells (2012)
  • Author(s): Watanabe M, Hida A, Kitamura S, Enomoto M, Ohsawa Y, Katayose Y, Nozaki K, Moriguchi Y, Aritake S, Higuchi S, Tamura M, Kato M, Mishima K
  • Journal Title: Biochem Biophys Res Commun Volume: 425(4) Pages: 902-907
  • DOI: 10.1016/j.bbrc.2012.08.008
  • Peer Reviewed
• [Presentation] 概日リズム睡眠障害患者の体内時計機能障害 (2013)
  • Author(s): 肥田昌子, 北村真吾, 大澤要介, 中崎恭子, 元村裕貴, 綾部直子, 片寄泰子, 野崎健太郎, 榎本みのり, 加藤美恵, 小野浩子, 亀井雄一, 三島和夫
  • Organizer: 第128回日本薬理学会関東部会
  • Place of Presentation: 東京(新宿区)
  • Year and Date: 2013-07-14
• [Presentation] 末梢時計機能は個人の概日/睡眠特性を反映する (2012)
  • Author(s): 肥田昌子, 北村真吾, 片寄泰子, 榎本みのり, 大澤要介, 渡邊真紀子, 野崎健太郎, 有竹清夏, 樋口重和, 加藤美恵, 守口善也, 亀井雄一, 池田政明, 三島和夫
  • Organizer: Neuroscience2012
  • Place of Presentation: 愛知(名古屋市)
  • Year and Date: 20120918-21
• [Presentation] 末梢時計リズムは個人の睡眠特性・生物時計機能を反映する (2012)
  • Author(s): 肥田昌子, 北村真吾, 大澤要介, 片寄泰子, 野崎健太郎, 榎本みのり, 有竹清夏, 樋口重和, 加藤美恵, 亀井雄一, 池田政明, 三島和夫
  • Organizer: 第19回日本時間生物学会学術大会
  • Place of Presentation: 北海道(札幌市)
  • Year and Date: 20120915-16
• [Presentation] 生体組織を利用したヒト生物時計機能評価-概日リズム睡眠障害患者への応用- (2012)
  • Author(s): 肥田昌子, 北村真吾, 大澤要介, 榎本みのり, 片寄泰子, 野崎健太郎, 加藤美恵, 守口善也, 亀井雄一, 三島和夫
  • Organizer: 日本睡眠学会第37回定期学術集会
  • Place of Presentation: 神奈川(横浜市)
  • Year and Date: 20120628-30