2014 Fiscal Year Final Research Report
Establishment of diagnosis based on molecular targeting imaging of cardiovascular diseases by scFv antibody against Tenascin-C
| Project/Area Number |
24791291
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | Mie University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ScFv型抗体 / テネイシン-C / 分子イメージング / 虚血性心疾患 |
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| Outline of Final Research Achievements |
Tenascin-C (TN-C) is expressed transiently inflammatory regions in various cardiovascular diseases. We investigated the possibility that specific antibody against TN-C is useful for detecting cardiovascular diseases as a molecular diagnostic reagent. Heavy chain variable region (VH) and light chain variable region (VL) genes of anti-TN-C antibody were obtained from total RNA in hybridoma, and were performed overlap-extended PCR. Expression vector inserted VH-linker-VL gene (scFv) was used in E. coli, in vitro cell free (RTS wheat germ) and mammalian cell line (HEK293) expression systems. As a result, the antibody expression was highly effective in HEK293 cell line system. As soon as it’s ready, we are planning to experiments of antibody accumulation in foci of myocarditis model mice.
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| Free Research Field |
抗体工学
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