2013 Fiscal Year Final Research Report
Radioresistancy by secreted factors from irradiated cells through mitochondrial functions
| Project/Area Number |
24791317
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | Oita University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 放射線治療生物学 / バイスタンダー効果 / ミトコンドリア / 活性酸素 / アスコルビン酸 / SOD2 |
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| Research Abstract |
We examined the mechanism of bystander effect through the secreted factors. We focused on the action of secreted factors to be involved in the mitochondrial function. After irradiation, conditioned medium containing secreted factors was transferred to another unirradiated cell and analyzed effects of these cells. We found the stimulation of permeability of mitochondrial membrane, which result in the reduction of the mitochondrial function, increase of reactive oxygen species, and gene mutation. These responses were suppressed by the treatment of ascorbic acid. In U251 cells but not MCF-7 cells, cells became radioresistant by the treatment of conditioned medium from irradiated cells. We concluded that the effects of secreted factors from irradiated cells can modulate mitochondrial function and this effect results in the change to the responses against further irradiation.
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