Elucidation of liver regeneration mechanisms using novel NASH animal model

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791401
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: The University of Tokyo
• Principal Investigator: INAGAKI Natsuko 東京大学, 分子細胞生物学研究所, 特任研究員 (00611419)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 肝臓学 / NASH / 肝再生

Research Abstract

Recently patients with non-alcoholic steatohepatitis (NASH) have been increased because of westernization of lifestyle in Japan, which evokes a serious problem in the society. The goal of the present study is to elucidate the difference in the regeneration mechanisms between NASH and normal livers using NASH animal models. Investigation for a long period observation of two NASH model mouse candidates proved that feeding of choline deficient amino acid diet to wild-type mice is appropriate to evoke NASH. Liver regeneration is significantly impaired in NASH liver compared with normal liver after 70% partial hepatectomy. Additionally the results by FACS analysis suggested the involvement of new blood cells in the delay of liver regeneration.

Research Products

• [Journal Article] Nephronectin is upregulated in acute and chronic hepatitis and aggravates liver injury by recruiting CD4 positive cells (2013)
  • Author(s): Inagaki FF, Tanaka M, Inagaki NF, Yagai T, Sato Y, Sekiguchi K, Oyaizu N, Kokudo N, Miyajima A
  • Journal Title: Biochem Biophys Res Commun Volume: 430 Pages: 751-756
  • Peer Reviewed
• [Journal Article] Suprachiasmatic nucleus : Cellular clocks and networks (2012)
  • Author(s): Honma S, Ono D, Suzuki Y, Inagaki N, Yoshikawa T, Nakamura W, Honma K
  • Journal Title: Prog Brain Res Volume: 199 Pages: 129-141
  • Peer Reviewed
• [Journal Article] Host cell species- specific effect of cyclosporine A on simian immunodeficiency virus replication (2010)
  • Author(s): Takeuchi H, Ishii H, Kuwano T, Inagaki N, Akari H, Matano T
  • Journal Title: Retrovirology Volume: 7 Pages: 90
  • Peer Reviewed
• [Presentation] 肝中皮細胞を用いた細胞療法の開発 (2014)
  • Author(s): 稲垣奈都子, 稲垣冬樹, 國土典宏, 宮島篤
  • Organizer: 第21回肝細胞研究会
  • Place of Presentation: 東京
  • Year and Date: 20140627-28
• [Presentation] 臨床応用に向けた肝中皮細胞シートの有用性の検討 (2014)
  • Author(s): 稲垣奈都子, 稲垣冬樹, 國土典宏, 宮島篤
  • Organizer: 第50回肝臓学会
  • Place of Presentation: 東京
  • Year and Date: 20140529-30
• [Presentation] 他家移植における肝中皮細胞シートの有用性の検討 (2014)
  • Author(s): 稲垣奈都子, 稲垣冬樹, 國土典宏, 宮島篤
  • Organizer: 第13回日本再生医療学会
  • Place of Presentation: 京都
  • Year and Date: 20140304-06
• [Presentation] 肝中皮細胞シートを用いた次世代型再生医療 (2013)
  • Author(s): 稲垣奈都子, 稲垣冬樹, 國土典宏, 宮島篤
  • Organizer: 第21回 JDDW(日本消化器関連学会週間)
  • Place of Presentation: 品川
  • Year and Date: 20131009-12
• [Presentation] 肝中皮細胞シートを用いた肝切除術後の癒着防止および再生促進療法の開発 (2013)
  • Author(s): 稲垣奈都子, 稲垣冬樹, 國土典宏, 宮島篤
  • Organizer: 第12回日本再生医療学会
  • Place of Presentation: 横浜
  • Year and Date: 20130321-23
• [Presentation] 肝炎におけるネフロネクチンの病態生理的意義 (2012)
  • Author(s): 稲垣冬樹, 田中稔, 稲垣奈都子, 國土典宏, 宮島篤
  • Organizer: 第19回肝細胞研究会
  • Place of Presentation: 札幌
  • Year and Date: 20120629-30
• [Patent(Industrial Property Rights)] 癒着防止材 (2014)
  • Inventor(s): 稲垣奈都子・稲垣冬樹・國土典宏・宮島篤
  • Industrial Property Rights Holder: 国立大学法人 東京大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: PCT/JP2014/51280
  • Filing Date: 2014-01-22
  • Overseas
• [Patent(Industrial Property Rights)] 中皮細胞の製造方法, 及び癒着防止用細胞シート (2014)
  • Inventor(s): 稲垣奈都子・稲垣冬樹・國土典宏・宮島篤
  • Industrial Property Rights Holder: 国立大学法人 東京大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 62/002, 448
  • Filing Date: 2014-05-25
  • Overseas