2013 Fiscal Year Final Research Report
Identification of the novel ASC-binding molecules as therapy targets for advanced colon cancer
| Project/Area Number |
24791405
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Shinshu University |
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Principal Investigator |
FUJII Chifumi 信州大学, 医学系研究科, 助教 (10361982)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | アダプタータンパク質 / シグナル伝達 |
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| Research Abstract |
Colorectal cancers have high rate of recurrence and is the second leading cause of death in Japan. ASC is an adaptor protein to form various inflammasomes, and has a crucial role for caspase-1 activation and secretion of IL-1b and IL-18 in innate immune cells. ASC has been also reported as an epigenetically silenced gene in several human cancers, including colon cancers. However, little information is available for the signaling pathway regulated by ASC in tumor cells. Here, in order to investigate the roles of ASC in cancer progression, we overexpressed ASC in two cancer cell lines. The cell proliferation was attenuated in ASC expressed cells. Using functional proteome approaches, we identified several candidate proteins involved in signal transduction, cell cycle, and apoptosis. Our results suggested that ASC might contribute to the suppression of cancer cell proliferation through a modulation of several signaling pathways.
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