2013 Fiscal Year Final Research Report
Characterization of hepatic cancer stem cells with billiary cell marker
| Project/Area Number |
24791409
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
KODAMA Sota 京都大学, 医学(系)研究科(研究院), その他 (10583875)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 再生医学 / 癌幹細胞 / 肝臓癌 |
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| Research Abstract |
In this study, we aimed to determine if cytokeratin 19 (CK19), a hepatic progenitor cell marker, can used as a CSC marker in HCC.
First, we transfected the CK19 promoter-driven enhanced green fluorescence protein gene into human HCC cell lines. Then we isolated CK19+ cells using fluorescence-activated cell sorting (FACS). Furthermore, xenotransplantation into non-obese diabetic/severe combined immunodeficiency mice revealed that CK19+ cells could reproduce themselves, differentiate into CK19- cells, and generate larger tumors at a higher frequency in vivo. These results indicated that CK19+ HCC cells possess CSC properties. And, immunohistochemistry analyses of 166 consecutive human HCC specimens resected at Kyoto University showed that CK19 expression is an independent predictor of postoperative recurrence.
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Research Products
(2 results)
