2013 Fiscal Year Final Research Report
Elucidation of the mechanism of IFNalpha/5-FU treatment by visualization of cell cycle
| Project/Area Number |
24791414
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
MAEDA Sakae 大阪大学, 医学部附属病院, 医員 (50597490)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | インターフェロン / ５FU / HCC / Cell cycle |
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| Research Abstract |
To study the role of IFNalpha in the induction of apoptosis of HCC cells, we transduced interferon-alpha-receptor-2(IFNAR2)into the human HCC cell line. Cell cycle was visualized by induction of Fucci system. Time lapse imaging for 72 hours after IFNalpha or 5-FU treatment showed that IFNalpha induced cell death at G0/G1 phase whereas 5-FU induced cell death at S/G2/M phase. Time lapse imaging after synchronization of cell cycle revealed that cell death induced by IFNalpha was observed at G0/G1 phase following after S/G2/M phase. This showed S/G2/M phase was active point for IFNalpha.
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