2013 Fiscal Year Final Research Report
Novel therapeutic strategy for liver regeneration focusing on local TGF-beta activation mechanism
| Project/Area Number |
24791434
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | TGF-β / トロンボスポンディン / 肝再生 |
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| Research Abstract |
We performed 70% hepatectomy in mice receiving normal saline, LSKL peptide, and sham operation.LSKL peptide was administered intraperitoneally at abdominal closure and 6 h after hepatectomy. Perioperative plasma TSP-1 levels were measured by ELISA system in patients with hepatectomy. LSKL peptide administration attenuated Smad2 phosphorylation at 6h. S-phase entry of hepatocytes was accelerated at 24 and 48h by LSKL peptide administration, and which resulted in faster recovery of the residual liver and body weight. H&E staining and blood biochemical examinations revealed no significant adverse effects occurred following the two LSKL peptide administrations. LSKL peptide during the early period after hepatectomy can promote liver regeneration. The transient inhibition of TSP-1/TGF-beta signal activation using LSKL peptide in the early period after hepatectomy may thus be a promising strategy to promote liver regeneration following hepatectomy.
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[Journal Article] Percentage of future liver remnant volume before portal vein embolization influences the degree of liver regeneration after hepatectomy2013
Author(s)
Okabe H, Beppu T, Nakagawa S, Yoshida M, Hayashi H, Masuda T, Imai K, Mima K, Kuroki H, Nitta H, Hashimoto D, Chikamoto A, Ishiko T, Yamashita Y, Baba H
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Journal Title
J Gastrointest Surg
Volume: 17(8)
Pages: 1447-51
DOI
Peer Reviewed
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[Journal Article] CD44s regulates the TGF-β-mediated mesenchymal phenotype and is associated with poor prognosis in patients with hepatocellular carcinoma2012
Author(s)
Mima K, Okabe H, Ishimoto T, Hayashi H, Nakagawa S, Kuroki H, Watanabe M, Beppu T, Tamada M, Nagano O, Saya H, Baba H
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Journal Title
Cancer Res
Volume: 1;72(13)
Pages: 3414-23
DOI
Peer Reviewed
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[Presentation] LSKL peptide inhibits thrombospondin-1- mediated TGF-β signal activation and accelerates liver regeneration after hepatectomy in mice2014
Author(s)
Kuroki H, Hayashi H, Nakagawa S, Sakamoto K, Higashi T, Nitta H, Hashimoto D, Chikamoto A, Beppu T, Baba H
Organizer
AACR Annual Meeting 2014
Place of Presentation
San Diego, California, USA
Year and Date
2014-04-07
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[Presentation] TSP-1 を介したTGF-β活性化による肝再生抑制機構をターゲットとした新規肝再生促進療法の開発2014
Author(s)
黒木 秀幸, 林 洋光, 中川 茂樹, 東 孝暁, 橋本 大輔, 阿部 真也, 新田 英利, 近本 亮, 石河 隆敏, 別府 透, 馬場 秀夫
Organizer
第114回日本外科学会定期学術集会
Place of Presentation
国立京都国際会館
Year and Date
2014-04-03
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[Presentation] TGF β活性化機構による肝再生抑制機構の解明と新戦略の構築2013
Author(s)
林 洋光, 黒木 秀幸, 美馬 浩介, 中川 茂樹, 新田 英利, 今井 克憲, 蔵元 一崇, 近本 亮, 別府 透, 酒井 尚雄, 馬場 秀夫
Organizer
第113回日本外科学会学術集会
Place of Presentation
マリンメッセ福岡
Year and Date
2013-04-11
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