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2014 Fiscal Year Final Research Report

Comprehensive analysis of PIK3CA-Akt pathway and miRNAs

Research Project

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Project/Area Number 24791449
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Digestive surgery
Research InstitutionNippon Medical School

Principal Investigator

AKAGI ICHIRO  日本医科大学, 医学部, 助教 (90552662)

Co-Investigator(Renkei-kenkyūsha) ISHIBASHI Osamu  大阪府立大学, 生命環境学研究科, 准教授 (70293214)
Project Period (FY) 2012-04-01 – 2015-03-31
KeywordsPI3K / Akt / miR-141 / miR-200 / DDIT4 / PHLPP2
Outline of Final Research Achievements

We focused on PI3K-AKT pathway and investigated comprehensively mRNA and miRNA expression in normal and tumorous human esophageal squamous cell lines. Using integrated statistical analyses, we identified miR-141, miR-200b, miR-200c, and miR-205 as highly up-regulated onco-miRs in esophageal squamous cell carcinoma (ESCC). According to the computational program TargetScan and our microarray-based comprehensive gene expression analysis, we detected DNA damage-inducible transcript 4 protein (DDIT4) as a target of miR-200b and miR-200c and which was downregulated in ESCC. Also, we detected PH Domain Leucine-Rich Repeat-Containing Protein Phosphatase 2 (PHLPP2) as a target for miR-141 and which was downregulated in ESCC. As expected, the transfection of miR-141 mimic resulted in significant decrease of the expression of PHLPP2 in esophageal epithelial cell line. These results suggest that miR-141 is a negative regulator of PHLPP2 and promotes cell proliferation in ESCC.

Free Research Field

食道外科

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Published: 2016-06-03  

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