2014 Fiscal Year Final Research Report
Elucidation of lumican and development of the new therapeutic drug for gastrointestinal neuroendocrine carcinoma
| Project/Area Number |
24791450
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 消化管神経内分泌癌 / がん幹細胞 / 細胞外基質 / lumican |
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| Outline of Final Research Achievements |
We hypothesized that NEC contains a subpopulation of cancer stem cells (CSCs), we examined expression and role of lumican and CSC marker in gastrointestinal neuroendocrine carcinoma (NEC). Cytoplasm and tumor stroma were positively stained for lumican by immunohistochemcal analysis. We used established cell line (NENMS-1) derived from ascending colon NEC cancer patient. Src，CD133 and lumican mRNA were over expressed in sphere than wild by quantitative RT-PCR analyses. Lumican-overexpressing NENMS-1 was reduced cell growth, sphere formation and cell migration compared with wild and Control-GFP. CD133 and lumican mRNA expression were high in positive compartmentation by aldefluor assay. From the above, it was suggested lumican may become a CSC marker for gastrointestinal NEC.
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| Free Research Field |
消化器外科
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