2013 Fiscal Year Final Research Report
Establishment of lung cancer cell line harboring acquired resistance to receptor tyrosine kinase inhibitor
| Project/Area Number |
24791482
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Thoracic surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
FUKUI Takayuki 名古屋大学, 医学部附属病院, 病院助教 (70463198)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 細胞株 / 肺癌 / 中皮腫 / 胸腺腫 |
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| Research Abstract |
We started primary culture using surgical specimens or pleural effusions from the patients with malignant tumors treated in Nagoya University. We could get surgical specimens from 20 patients in two years and succeeded in the establishment of the cell lines from a mesothelioma and 3 thymic tumor cases. However, We can not able to establish a cell line harboring acquired resistance to epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) which I planned since we could not get the specimens from the patients who were resistant to EGFR-TKI. Thus, we could not able to analyze any gene alterations associated with the EGFR gene using those cell lines. However, several cell lines from mesothelioma and thymoma was successfully primary cultured and established. The cell lines from thoracic malignant tumors except the lung cancer was relatively rare and we performed immunostaining or the graft making with the mouse and confirmed the establishment of the cell lines.
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Research Products
(5 results)
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[Presentation] 胸腺腫細胞株樹立の試み2014
Author(s)
石黒太志, 谷口哲郎, 宇佐美範恭, 川口晃司, 福井高幸, 中村彰太, 尾関直樹, 加藤毅人, 山谷千尋, 横井香平
Organizer
第33回日本胸腺研究会
Place of Presentation
東京大学(東京都文京区)
Year and Date
2014-02-08
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