2013 Fiscal Year Final Research Report
Activated microglia in a rat stroke model express NG2 proteoglycan in peri-infarct tissue through the involvement of TGF-beta1
| Project/Area Number |
24791507
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka University (2013)
Ehime University (2012) |
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Principal Investigator |
SUGIMOTO Kana 大阪大学, 医学(系)研究科(研究院), 助教 (00581034)
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| Co-Investigator(Renkei-kenkyūsha) |
TANAKA Junya 愛媛大学, 大学院・医学系研究科, 教授 (70217040)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 脳梗塞 / NG2コンドロイチン硫酸プロテオグリカン / マイクログリア / TGF-β1 |
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| Research Abstract |
We investigated activated microglia in ischemic brain lesions. Activated microglia expressing NG2 chondroitin sulfate proteoglycan (NG2) were found only in the narrow zone (demarcation zone) that demarcated the peri-infarct tissue and ischemic core. NG2(+) microglia expressed both CD68 and a TREM-2, suggesting that NG2(+) microglia eliminated apoptotic neurons. In fact, NG2(+) microglia often attached to degenerating neurons and sometimes internalized NeuN(+) or neurofilament protein(+) material.Kinetic studies revealed that expression of transforming growth factor (TGF)-beta1 was most evident in the ischemic core. In response to TGF-beta1, primary microglia enhanced the expression of NG2 protein and TREM-2 mRNA as well as migratory activity.A TGF-beta1 inhibitor, SB525334, abolished these effects. The present results suggest that TGF-beta1 produced in the ischemic core diffused toward the peri-infarct tissue, driving activated microglial cells to eliminate degenerating neurons.
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[Journal Article] Expression of MCP-1 and Fractalkine on Endothelial Cells and Astrocytes May Contribute to the Invasion and Migration of Brain Macrophages in Ischemic Rat Brain Lesions2013
Author(s)
Nari Tei, Junya Tanaka, Kana Sugimoto, Tasuku Nishihara, Ryutaro Nishioka, Hisaaki Takahashi, Hajime Yano, Shirabe Matsumoto, Shiro Ohue, Hideaki Watanabe, Yoshiaki Kumon, Takanori Ohnishi
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Journal Title
Journal of Neuroscience Research
Volume: 91
Pages: 681-693
Peer Reviewed
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