2013 Fiscal Year Final Research Report
Pathological mechanism of motion disorders associated with CRPS type II. An animal study
| Project/Area Number |
24791537
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | CRPS / 関節拘縮 / NGF |
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| Research Abstract |
Ten-week-old male Wistar rats were assigned to 5 groups: a normal group receiving no treatment; a sham operation group with exploration of the left L5 nerve root, femur, and tibia; an immobilization group with surgical exploration plus internal knee joint immobilization; a surgical neuropathy group prepared by spinal nerve ligation (SNL) of the left L5 nerve root; and a surgical neuropathy + immobilization group with simultaneous SNL and knee joint immobilization. Mechanical allodynia and knee contracture were compared between groups, and tissues were harvested for histological assessments and gene and protein expression analyses. Neither surgical procedures nor immobilization induced detectable mechanical hypersensitivity, while addition of nerve injury resulted in detectable mechanical allodynia. Nerve growth factor (NGF) and other mediators of neurogenic inflammation were highly expressed not only in denervated muscles, but also in innervated muscles in contiguous areas.
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