2013 Fiscal Year Final Research Report
Modeling type II collagenopathy
| Project/Area Number |
24791541
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
OKADA Minoru 京都大学, iPS細胞研究所, 研究員 (30625835)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUMAKI Noriyuki 京都大学, iPS細胞研究所, 教授
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 整形外科学 / 細胞生物学 / 分子生物学 |
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| Research Abstract |
The purpose is to clarify mechanisms of type II collagenopathy and to find drugs for medical application. In this study, we established disease models of type II collagenopathy using cell reprogramming technologies. To generate disease-specific chondrocytes, we introduced retrovirus or plasmid vectors encoding specific genes to patients fibroblasts. Disease-specific chondrocytes derived from patients fibroblasts or iPS cells showed intracellular accumulation of mutated type II collagen and endoplasmic reticulum stress, resulting apoptosis. These disease models reflect phenotypes of type II collagenopathy and can be useful for drug screening in further investigations.
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