2014 Fiscal Year Final Research Report
Development of novel fracture healing methods using growth factor containing a collagen-binding domain from Clostridium histolyticum collagenase
| Project/Area Number |
24791560
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | コラーゲン結合型成長因子 / コラーゲン / 脱灰骨 / 骨折治癒 |
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| Outline of Final Research Achievements |
We attempted to develop a collagen-based bone formation system using a fusion protein (collagen binding-bFGF, CB-bFGF) consisting of bFGF and the collagen-binding domain (CBD) of Clostridium histolyticum collagenase. The affinity of the fusion protein towards collagen and demineralized bone powder (DBP) was confirmed by collagen-binding assays. Moreover, the combination of the collagen binding-bFGF fusion protein (CB-bFGF) with collagen powder or DBP induced bone formation at protein concentrations lower than those required for bFGF alone in mice fracture models. Therefore, because of the potential adverse effects of bFGF, particularly when administered at high doses, the use of CP/CB-bFGF composite may be a safer alternative for bone repair by limiting bFGF exposure at fracture sites. Taken together, these properties suggest that the CB-bFGF/collagen powder composite is a promising injectable material for fracture healing in the clinical setting.
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| Free Research Field |
整形外科学
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