2015 Fiscal Year Final Research Report
Mechanisms of intervertebral disc degeneration in postmenopausal women
| Project/Area Number |
24791562
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | National Defense Medical College (2013-2015)
Keio University (2012) |
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Principal Investigator |
Hosogane Naobumi 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, その他部局等, 助教 (10365306)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 椎間板 / 退行変性 / 活性酸素 |
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| Outline of Final Research Achievements |
Intervertebral disc (IVD) degeneration was accelerated in ovariectomy rats, although the phenotype was not obvious enough to perform further analysis. Therefore, IVD degeneration was induce by disc puncture with needle and revealed that oxidative stress was involved in the degeneration. mRNA expression of catabolic factors such as TNF-alpha, MMP-3, COX-2 were increased in degenerated IVD of punctured rats. In vitro study, oxidative stress induced with H2O2 upregulated the expressions of these catabolic factors. Finally, degeneration of IVD induced with oxidative stress was rescued with administration of antioxidant NAC (N-acteyl cysteine) both in vivo and in vitro analysis.
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| Free Research Field |
脊椎
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