Analysis of pathological role of cytoplasmic oxidative stress in disuse bone atrophy.

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791569
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Juntendo University
• Principal Investigator: NOJIRI Hidetoshi 順天堂大学, 医学部, 助教 (10317456)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 骨・軟骨代謝学 / 廃用 / 骨萎縮 / 酸化ストレス / SOD1

Research Abstract

We herein report that mechanical unloading significantly increased intracellular ROS production and the specific up-regulation of Sod1 in bone tissue in a tail-suspension experiment. We also reveal that Sod1 loss exacerbated bone loss via reduced osteoblastic abilities during mechanical unloading. We found that the administration of an antioxidant, vitamin C, significantly attenuated bone loss during unloading. These results indicate that mechanical unloading, in part, regulates bone mass via intracellular ROS generation and the Sod1 expression.

Research Products

• [Journal Article] Cytoplasmic reactive oxygen species and SOD1 regulate bone mass during mechanical unloading (2013)
  • Author(s): Morikawa D, Nojiri H, Saita Y, Kobayashi K, Watanabe K, Ozawa Y, Koike M, Asou Y, Takaku T, Kaneko K, Shimizu T
  • Journal Title: J Bone Miner Res Volume: 28 Pages: 2368-80
• [Presentation] Analysis of oxidative stress and antioxidant enzymes in mechanical stress response (2011)
  • Author(s): Morikawa D, Saita Y, Nojiri H, Kobayashi K, Watanabe K, Asou Y, Kaneko K, Shimizu T
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: Gold Coast, Australia
  • Year and Date: 20110904-08
• [Presentation] メカニカルストレス応答における酸化ストレスと抗酸化酵素の解析 (2011)
  • Author(s): 森川大智, 野尻英俊, 斎田良知, 小林慶司, 渡辺憲史, 麻生義則, 金子和夫, 清水孝彦
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 20110700