2013 Fiscal Year Final Research Report
The mechanosensor Piezo1 is expressed in the urothelium and has a crucial function in the urine storage phase in the bladder.
| Project/Area Number |
24791640
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAGOMI Hiroshi 山梨大学, 医学部附属病院, 助教 (80418714)
TAKEDA Masayuki 山梨大学, 医学工学総合研究部, 教授 (80197318)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | piezo1 |
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| Research Abstract |
We provide evidence for a critical role of the Piezo1 channel in bladder function.We performed a systematic analysis of the molecular and functional expression of Piezo1 channels in the urothelium.Immunofluorescence demonstrated that Piezo1 was abundantly expressed in mouse urothelium.Intracellular Ca2+ measurements in urothelial cells isolated from mice revealed a Piezo1-dependent response to mechanical stretch stimuli.GsMTx4, a known inhibitor of stretch-activated channels, attenuated the response to Ca2+ influx in urothelial cells upon stretch stimulation experiments.Behavioral studies demonstrated that GsMTx4-treated mice exhibited increased bladder capacity and reduced micturition frequency compared with the vehicle-treated group. These results suggested that Piezo1 sensed distention of the bladder urothelium during urine storage. Furthermore, inhibition of Piezo1 may provide a promising means of treating bladder dysfunction.
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