2014 Fiscal Year Final Research Report
The breakthrough of male infertily by stem cell research
| Project/Area Number |
24791674
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | Toho University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | 男性不妊 / 幹細胞 / iPS |
|---|
| Outline of Final Research Achievements |
Klinefelter syndrome (KS) is associated with infertility due to non-obstructive azoospermia (NOA). The mechanism underlying NOA is still poorly understood. The generation of induced pluripotent stem (iPS) cells derived from KS patients may be useful for studying the disease mechanism and identifying novel therapies. Cells from KS patients were transduced with Sendai viral vectors encoding four transcription factors, OCT4, SOX2, KLF4, and C-MYC, and the transduced cells were analyzed for in vitro and in vivo pluripotency. KS patient-derived iPS cells were successfully generated and shown to produce teratomas in the testes of SCID mice. In vitro cardiac differentiation of the iPS cells was confirmed by the presence of clusters of beating cells.High quality KS patient-derived iPS cells with cardiomyocyte-differentiating ability were established.
|
| Free Research Field |
男性不妊
|
