New method for non-invasive diagnosis and treatment of bladder tumor based on the analysis of urine microRNA

2014 Fiscal Year Final Research Report

• Project/Area Number: 24791676
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: SASAKI Hideo 聖マリアンナ医科大学, 医学部, 講師 (30386990)
• Research Collaborator: OCHIYA Takahiro 国立がんセンター, がん転移研究室, 室長 ; KOSAKA Nobuyosi 国立がんセンター, がん転移研究室, 研究員 ; NOZAWA Shiari 聖マリアンナ医科大学, 腎泌尿器外科, 講師 ; YOSHIIKE Miki 聖マリアンナ医科大学, 腎泌尿器外科, 研究技術員
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 膀胱腫瘍 / microRNA / 腫瘍マーカー

Outline of Final Research Achievements

This study aimed to establish new urine markers for diagnosis of bladder tumor. On the basis of miRNA microarray analyses of total RNA isolated from bladder tumor cell lines, we found three candidate miRNAs (miR-A, B, and C) that were up-regulated in bladder tumor cells. The determination of the levels of these miRNAs in the bladder washings from patients with or without bladder tumor revealed that miR-A was significantly up-regulated in the patients with bladder tumor, and able to discriminate the tumor group from normal one by setting an appropriate cut-off value. Comparable results were also obtained in voided urine samples from the patients, and furthermore, miR-A in urine discriminated the bladder tumor patients negative for urine cytology. The expression levels of miR-A correlated with both pathological grade and stage, and normalized after tumor resection, which suggest that it is a promising urine marker specific for bladder tumor.

Free Research Field

泌尿器科学