Evidence of the activation of unfolded protein response in granulosa and cumulus cells during follicular growth and maturation

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791689
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: The University of Tokyo
• Principal Investigator: HARADA Miyuki 東京大学, 医学部附属病院, 助教 (70451812)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 卵巣 / 小胞体ストレス / 卵胞発育 / 卵胞成熟 / 顆粒膜細胞

Research Abstract

Follicular growth and maturation are known to be regulated by intra-ovarian factors, although the mechanisms are still to be known. Recently, unfolded protain response (UPR) has been known to have essential roles in keeping homeostasis. In the present study, to test whether UPR in granulosa cells (GC) and cumulus cells (CC) is involved in the process of follicular growth and maturation, we examined the presence of UPR in GC during follicular growth and the association between UPR expression in CC and oocyte maturity. UPR-related genes, spliced form of X-box binding protein -1 (XBP1(S)) and heat shock 70kDa protein 5 (HSPA5), were expressed in mice GC of growing follicels, and their expression is stage-dependent. In addition, the expression levels of XBP1(S) mRNA in CC enclosing human mature oocytes were 2-fold higher than those in CC enclosing immature ooytes. These findings suggest that UPR is activated in GC during follicular growth, playing roles in follicular growth and maturation.

Research Products

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  • Author(s): Akiyama I, Yoshino O, Osuga Y, Shi J, Harada M, Koga K, Hirota Y, Hirata T, Fujii T, Saito S, Kozuma S
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  • DOI: 10.1210/jc.2012-3475
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• [Journal Article] (2012)
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• [Journal Article] Successful pregnancy following low-dose hCG administration to hMG in a patient with hypothalamic amenorrhea due to weight loss (2012)
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