PATHOLOGICAL INVESTIGATION OF PLACENTA PREVIA ACCRETA: THE SITE OF UTERINE WALL AND THE WIDTH/DEPTH OF VILLOUS INVASION

2015 Fiscal Year Final Research Report

• Project/Area Number: 24791699
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Nagoya University
• Principal Investigator: SUMIGAMA SEIJI 名古屋大学, 医学(系)研究科(研究院), 特任講師 (50378121)
• Research Collaborator: WATANABE Yuriko 中部ろうさい病院, 産婦人科, 副部長 ; HATTORI Yuka 名古屋掖済会病院, 産婦人科, 医長 ; MATSUKAWA Satoshi 小牧市民病院, 産婦人科, 医長
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 癒着胎盤 / 血清マーカー

Outline of Final Research Achievements

The objective of the current study is to examine molecular biological change of trophoblast invasion in placenta accreta by DNA microarray analysis. We isolated villous trophoblast from invasive site and control from non-invasive site in a case of placenta accreta and extracted complemantary DNA by RT-PCR. A DNA microarray analysis and Gene ontology analysis extracted overexpressed genes, MMP-19, ADAM-28, cathepsin S and cathepsin L. Immunohistochemistry of accreta cases revealed poor expression of MMP-19 and no difference between invasion and non-invasion site in ADAM-28 and cathepsin S, however a abundant expression of cathepsin L in especially in extra-villous trophoblast in invasion site. The serum cathepsin L level in placenta previa with accreta was significantly higher than those of normal pregnancy and placenta previa with no accreta. Thus, our data suggests a role for cathepsin L in the regulation of abnormal invasion of trophoblast in case of placenta accreta.

Free Research Field

産婦人科