2013 Fiscal Year Final Research Report
Search for the genes involved in the development of uterine leiomyoma
| Project/Area Number |
24791704
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
ASADA Hiromi 山口大学, 医学部附属病院, 助教 (90526906)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 生殖医学 |
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| Research Abstract |
Profiles of genome-wide DNA methylation and mRNA expression were investigated in leiomyomas and in myometrium with and without leiomyomas. We identified 625 genes whose DNA methylation and mRNA expression patterns differed between leiomyomas and the adjacent myometrium. We transduced 3 genes (NRG1, NR3C1 and MAPK10) of 76 genes we identified by upstream factor analysis each in lentivirus-vectors. We will transduce these vectors in immortalized human uterine smooth muscle cell and transplant them under the kidney capsule in Immunodeficient mice.
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