2014 Fiscal Year Final Research Report
Functional analysis of cancer stem cell marker EpCAM in ovarian cancer
| Project/Area Number |
24791713
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 卵巣癌 / 癌幹細胞 / 抗癌剤治療抵抗性 |
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| Outline of Final Research Achievements |
The present study was designated to evaluate the role of Epithelial cell adhesion molecule (EpCAM) in tumor resistance to chemotherapy in association with ovarian cancer stem cell.
To investigate whether the EpCAM-positive subpopulations enhance chemoresistance, ovarian cancer cells were exposed to cisplatin in vitro. Flow cytometric analysis showed that treatment with cisplatin result in enhanced expression of EpCAM in residual cells as compared to untreated cells. To evaluate the in vivo relevance of chemoresistance in EpCAM-positive cells, we studied an established mouse model in vivo, and we found that EpCAM-positive ovarian cancer cells are involved in resistance to chemotherapy. Given that a distinct subpopulation of EpCAM-positive cancer cells plays a critical role in chemoresistance, definitive treatment should target this subpopulation of EpCAM-positive cells in ovarian cancer.
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| Free Research Field |
婦人科学
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